BlastProfiler: a sequence profile generator
Parse and clean up Psi-Blast output to generate a representive sequence profile
INPUT VALUES
Email
Paste your protein sequence in the window ... (use one letter codes)
OR
upload your sequence file
Perform a PSI-BLAST search?
Database
nr
pdb
Iteration
1
2
3
4
OR
upload PSI-BLAST output file
SEQUENCE HIT FILTERING
Minimum sequence identity between each hit and query
% (default: 30)
Maximum sequence identity between each hit and query
% (default: 90)
Maximum e-value for each hit
(default: 1x10
-4
)
Minimum percentage of sequence overlap between each hit and query
% (default: 75)
Minimum length of sequence overlap between each hit and query
residues (default: 30)
Maximum sequence identity between pairs of aligned sequence hits
% (range: 40-100; default: 90)
OUTPUT OPTIONS
Raw sequences
Format
FASTA
PIR
Number of columns for alignment output
(default: 70)
Alignment
Format
FASTA
PIR
CLUSTALW
Number of columns for alignment output
(default: 70)
Realign sequences with CLUSTALW
Format
CLUSTALW
FASTA
PIR
PHYLIP